《现代养殖设施与设备》教材建设探索与实践     

马伟  黄炎坤  程璞  徐秋良  郑立  曾照烨 《畜牧与饲料科学》2018,39(9):77-80

针对《现代养殖设施与设备》课程没有规范教材的现状，探索符合人才培养目标和规格、适应市场需求的特色应用型教材建设，为应用型人才培养提供知识载体。并通过进一步实践，为教材的修订完善明确了方向。  相似文献   

Long‐term effects of organic manure and manufactured fertilizer additions on soil quality and sustainable productivity of finger millet under a finger millet–groundnut cropping system in southern India          下载免费PDF全文

A. Sathish  B. K. Ramachandrappa  M. A. Shankar  P. N. Srikanth Babu  CH. Srinivasarao  K. L. Sharma 《Soil Use and Management》2016,32(3):311-321

In a 20‐yr‐old long‐term experiment, the impact of continuous application of organic manures and inorganic fertilizers on soil quality and the sustainability of finger millet production was conducted on two cropping systems: finger millet and finger millet–groundnut on an Alfisol of semi‐arid southern India. The study was conducted from 1992 to 2011 at the All India Coordinated Research Project for Dryland Agriculture, UAS, Bangalore, using a randomized block design. The treatments comprised of T₁: control [no fertilizer and no farmyard manure (FYM) applied], T₂: FYM 10 t/ha, T₃: FYM 10 t/ha + 50% of recommended NPK (50:50:25 kg/ha), T₄: FYM 10 t/ha + 100% of recommended NPK and T₅: 100% recommended NPK. Comparison of long‐term yield data between treatments was used to calculate a ‘sustainability yield index’ (SYI), which was greatest for T₄ (FYM 10 t/ha + 100% of recommended NPK), in both rotational (0.68) and monocropping (0.63) situations. Soil quality indices were determined using principal component analysis linear scoring functions. The key indicators which contributed to the soil quality index (SQI) under rotation were organic C; potentially available N; extractable P, K and S; exchangeable Ca and Mg; dehydrogenase activity and microbial biomass C and N. The largest SQI (7.29) was observed in T₄ (FYM 10 t/ha + 100% NPK), and the smallest (3.70) SQI was for the control. Application of 10 t/ha FYM together with NPK (50:50:25 kg/ha) sustained a mean yield of 3884 kg/ha.  相似文献   

Clinical peripheral neuropathy associated with diabetes mellitus in 3 dogs     

Morgan MJ  Vite CH  Radhakrishnan A  Hess RS 《The Canadian veterinary journal. La revue veterinaire canadienne》2008,49(6):583-586

Clinical and electrodiagnostic findings in 3 spontaneously diabetic dogs with clinical peripheral neuropathy (PN) are reported. Clinical signs of a PN may develop in diabetic dogs with adequate glycemic control. In addition, laryngeal paralysis may develop in association with diabetes mellitus in dogs with clinical PN.  相似文献   

CT-guided stereotactic brain biopsy using the Kopf stereotactic system     

Mark T Troxel  Charles H Vite 《Veterinary radiology & ultrasound》2008,49(5):438-443

This report describes computed tomography (CT)-guided stereotactic brain biopsy using the Kopf stereotactic system, a commercially available patient restraint system which does not require additional modification for use in small animals. The accuracy of biopsy needle placement was determined by injecting dilute iohexol into cadaver brains and comparing the three-dimensional coordinates of the desired target location to the actual needle tract observed on postcontrast CT images. Overall mean error in needle placement in a dorsoventral trajectory was 0.9 +/- 0.9 mm (n = 80 injections) for dogs and 1.0 +/- 1.1 mm (n = 30 injections) for cats. The overall mean error in needle placement via an oblique trajectory in five dogs was 1.7 +/- 1.6 mm (n = 12 injections). These results suggest that this system can be used to successfully place a biopsy needle into the brain to obtain biopsies from small lesions.  相似文献   

Loss of Restriction Site DdeI, used for Avian Molecular Sexing, in Oreophasis derbianus     

LG Bermúdez-Humarán  P Chávez-Zamarripa  A Guzmán-Velasco  CH Leal-Garza  R Montes de Oca-Luna 《Reproduction in domestic animals》2002,37(6):321-323

Molecular sexing is a rapid and safe procedure for bird sex determination. Two universal methods based on the amplification of a chromo‐helicase‐DNA‐Binding 1 (CHD) gene region, located in both sexual chromosomes (Z and W), have been established. We found that molecular sexing of Oreophasis derbianus failed by using these two procedures. One of them is based on a restriction site located in CHD1W gene but absent in CHD1Z. The DdeI restriction site, used successfully to determine gender in several bird species, was found to be lost because of nucleotide change in O. derbianus. This change created a new restriction site, NlaIII, that was successfully applied for sexing this endangered bird.  相似文献   

Fibrin–alginate hydrogel supports steroidogenesis,in vitro maturation of oocytes and parthenotes production from caprine preantral follicles cultured in group          下载免费PDF全文

IR Brito  GM Silva  AD Sales  CH Lobo  GQ Rodrigues  RF Sousa  AAA Moura  CEM Calderón  M Bertolini  CC Campello  J Smitz  JR Figueiredo 《Reproduction in domestic animals》2016,51(6):997-1009

This study aimed to establish a culture system that improves the in vitro development of caprine preantral follicles. In a first experiment, follicles were encapsulated as a single unit per bead and cultured singly or in groups or with five follicles in the same alginate (ALG) bead for 18 days. In a subsequent experiment, the “five follicles per bead” design was chosen to culture in ALG, fibrin–alginate (FA) or hyaluronate (HA) for 18 days. In a third experiment, we chose the five follicles per bead in FA to culture for 30 days. The culture set‐up of five follicles per ALG bead increased antrum formation and follicle diameter compared to the other culture designs (p < .05). Moreover, under this condition, 44.44% of the oocytes from in vitro cultured preantral follicles reached meiotic resumption. A significant increase of follicle diameter occurred in attachment system and FA (p < .05), but the ALG condition reached the highest among all groups on day 18 (p < .05). Follicles encapsulated in matrix produced more estradiol and progesterone than attachment system (p < .05). The expression of MMP‐9 mRNA was higher in FA than in other groups (p < .05) and similar to antral follicles from in vivo control (p > .05). Only FA group resulted in oocytes matured. After 30 days, oocytes from preantral follicles in vitro grown in FA developed to eight‐cell parthenotes. In conclusion, a culture system using FA supported the development of caprine preantral follicles cultured in group and included in the same bead of hydrogel, improving the oocyte maturation and producing parthenotes.  相似文献   

Molecular characterization of an influenza A virus (H4N2) isolated from waterfowl habitats in the State of Mexico     

Erika ORNELAS-EUSEBIO  Alejandro OBREGóN-ASCENCIO  Fernando CHáVEZ-MAYA  Gary GARCíA-ESPINOSA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(3):365-369

Wild waterfowl and their habitats are the main reservoirs of influenza A virus (IAV) mainly during the breeding season and prior to migration. This study describes the molecular characterization of an IAV isolated from 240 water samples of a small wetland during non-breeding season of migratory wild ducks in the State of Mexico, Mexico. The results showed that the virus belongs to the H4N2 subtype and each of its eight segments of the viral genome has similarity to IAV isolated from ducks in North America. This study suggests that IAV can be isolated from small wetland during non-breeding season of migrating waterfowl.  相似文献   

MRI and electrophysiological abnormalities in a case of canine globoid cell leucodystrophy     

F. Cozzi    C. H. Vite    D. A. Wenger  T. Victoria  M. E. Haskins  † 《The Journal of small animal practice》1998,39(8):401-405

A six-month-old West Highland white terrier with progressive, multifocal neurological disease was diagnosed with canine globoid cell leucodystrophy (GCL). Magnetic resonance imaging (MRI) of the brain was performed, as well as electrophysiological testing (including brainstem auditory evoked response, peripheral nerve conduction velocity, repetitive stimulation, F wave analysis and electromyography). MRI findings were consistent with diffuse, symmetrical white matter disease. Electrodiagnostic testing revealed evidence of peripheral neuropathy and an abnormal brainstem auditory evoked response. These observations were consistent with the pathological changes in central and peripheral white matter described for canine GCL, and resembled what has been described in human patients. It is believed that the tests may raise the suspicion of GCL in dogs and may aid in monitoring disease progression.  相似文献   

Solarization for the control of Pueraria montana (kudzu)     

CH NEWTON  LR NELSON  SJ DEWALT  EA MIKHAILOVA  CJ POST  MA SCHLAUTMAN  SK COX  WC BRIDGES  KC HALL 《Weed Research》2008,48(5):394-397

The effects of translucent polyethylene sheeting as a thermal covering to eradicate Pueraria montana (kudzu) were investigated at Clemson, South Carolina on a clay loam. In 2005, the highest reduction of live root crowns was observed where P. montana was covered for the entire growing season (May–October) with a reduction of 42% of live root crowns compared with the control plots. Where P. montana was covered for alternate weeks throughout the growing season live root crowns were reduced by 35%. Covering P. montana for one week and then uncovering for four weeks repeatedly through the season killed 24% of root crowns. In 2006, the second year of treatment to the same plots, the three solarization treatments had similar efficacy of about 97%. The use of polyethylene sheeting appears not to be cost‐effective for general control of large P. montana infestations, but may be useful for small patches.  相似文献   

Prevalence of diseases of the spinal cord of cats     

Marioni-Henry K  Vite CH  Newton AL  Van Winkle TJ 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2004,18(6):851-858

A retrospective review of records of 205 cats with histologically confirmed disease of the spinal cord was performed to identify the prevalence of disease in this nonrandomly selected population of cats. Clinical records were reviewed, and age, duration of neurologic illness, and clinical and histopathologic findings in cats with spinal cord disease were abstracted. Disease processes were classified into 7 categories and 23 groups. The most common diseases affecting the spinal cord of cats were feline infectious peritonitis (FIP), lymphosarcoma (LSA), and neoplasia of the vertebral column secondarily affecting the spinal cord. Information on age, onset and duration of clinical signs, and lesion localization at the postmortem examination in cats belonging to the 7 categories of disease were analyzed to create a practical list of differential diagnoses. Cats were also subcategorized into 3 groups based on their age at death. FIP was the most common disease of cats younger than 2 years of age. LSA and vertebral column neoplasia were the most common diseases affecting cats between 2 and 8 years of age. Vertebral column neoplasia was the most common disease affecting cats older than 8 years of age. Results of this histopathologic study showed that FIP and LSA were the most common disease processes affecting the spinal cord of cats. However, at least 21 other groups of diseases and their relative prevalence were identified.  相似文献